Green synthesis of silk sericin-embedded silver nanoparticles and their antibacterial application against multidrug-resistant pathogens

Abstract Background The green synthesis strategy of metallic nanoparticles (NPs) has become popular due to being environmentally friendly. Stable silver (AgNPs) have been synthesized by natural products such as starch, soy protein, various extract leaves, barks, and roots functioning both reducing stabilizing agents. Likewise, silk sericin (SS) is a globular protein discarded in the factory might be used for NP synthesis. In this research, we focus on stabilization AgNPs SS well assessment their antibacterial activities against some drug-resistant pathogen. Results was extracted from Bombyx mori silkworm cocoons an aqueous medium. 17 w/w% dry powder with respect cocoon’s weight obtained freeze-drying. Furthermore, conjugated sericin, i.e., SS-capped (SS-AgNPs) were easy, cost-effective, environment-friendly methods. SS-AgNPs characterized UV-visible spectroscopy, Fourier-transform infrared-attenuated total reflection (FTIR-ATR) transmission electron microscopy (TEM), X-ray diffraction measurement. It found absorbance spectroscopy that higher percent at concentration nitrate solution. FTIR-ATR spectra showed carboxylate groups act agent nanoparticles, while NH 2 + COO? stabilizer AgNPs. diffractogram quite different AgNO 3 change crystal structure. diameter around 20–70 nm observed using TEM. exhibited strong activity multidrug-resistant pathogens, Escherichia coli Pseudomonas aeruginosa . Minimal inhibitory/bactericidal concentrations E. P. 20?g/mL. Conclusions This study encourages use ecofriendly control microorganisms.